Journal of Phytopathology - Phytopathologische Zeitschrift (2003) 151, 290-296

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H.-J. Su, L.-Y. Tsao, M.-L. Wu and T.-H. Hung (2003)
Biological and molecular categorization of strains of Banana bunchy top virus
Journal of Phytopathology - Phytopathologische Zeitschrift 151 (5), 290-296
Abstract: Banana bunchy top virus (BBTV), a complex circular single-stranded DNA virus with multiple genomic components, is a destructive pathogen in banana-cultivating areas worldwide. Based on symptoms (such as vein clearing, green streak on pseudostem, leaf atrophy, bunchy top and stunting) as well as polymerase chain reaction (PCR) amplification patterns with different primer pairs, all BBTV isolates collected from Taiwan and other countries can be divided into five distinctive strains. Three primer pairs, C1, stem-loop common region (CR-SL) and TS were used for PCR amplifications. Strain 1, which induces conspicuous symptoms, is a common severe strain; it reacted positively with C1 and CR-SL but negatively with TS in the PCR assays, so its PCR pattern was indicated as '+/+/-' for C1, CR-SL and TS primer pairs, respectively. Strain 2 seemed to be a Taiwan-specific severe strain which induced severe symptoms, and its PCR pattern was '+/+/+' as it showed positive reactions with all three primer pairs. Strain 3, causing the most severe symptoms, is a Malaysia-specific severe strain whose PCR pattern is '-/+/-'. Strain 4 induced moderately severe symptoms and is an intermediate strain whose PCR pattern is '-/+/-'. Strain 5 is a mild strain; it did not induce symptoms in banana and it reacted positively with C1, CR-SL and TS primer pairs. Interestingly, an additional 537-bp fragment was amplified from Strain 5 with the CR-SL primer pair. The PCR pattern of Strain 5 is therefore indicated as '+/++/+'. This study demonstrates that various BBTV strains exist in nature and they differ biologically and also molecularly.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ting-Hsuan Hung

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Banana bunchy top virus Taiwan
Banana bunchy top virus Banana/plantain (Musa) Malaysia
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